USNS Comfort (T-AH-20) is the third United States Navy ship to bear the name Comfort, and the second Mercy-class hospital ship to join the U.S. Navy's fleet. The USNS prefix identifies the Comfort as a non-commissioned ship owned by the U.S. Navy and operationally crewed by civilians from the Military Sealift Command (MSC). A uniformed naval hospital staff and naval support staff is embarked when Comfort is deployed, said staffs consisting primarily of naval officers from the Navy's Medical Corps, Dental Corps, Medical Service Corps, Nurse Corps and Chaplain Corps, and naval enlisted personnel from the Hospital Corpsman rating and various administrative and technical support ratings (e.g., Yeoman, Personnel Specialist, Information Systems Technician, Religious Program Specialist, etc.).

In keeping with her status as a non-combatant vessel, naval personnel from the "combat" specialties are not assigned as regular crew or staff. Underway embarks by Navy Unrestricted Line officers (e.g., warfare qualified combat specialties), enlisted Naval Aviation, Surface Warfare, Submarine Warfare, Special Operations or Special Warfare/SEAL personnel, or any Marine Corps officers or enlisted personnel, are typically limited to official visits, helicopter or tilt-rotor flight operations or as patients.

In accordance with the Geneva Conventions, USNS Comfort and her crew do not carry any offensive weapons. Firing upon Comfort would be considered a war crime as the ship only carries weapons for self-defense.